Atolypenes, tricyclic bacterial sesterterpenes discovered using a multiplexed in vitro Cas9-TAR gene cluster refactoring approach

Most natural product biosynthetic gene clusters identified in bacterial genomic and metagenomic sequencing efforts are silent under laboratory growth conditions. Here, we describe a scalable biosynthetic gene cluster activation method wherein the gene clusters are disassembled at interoperonic regio...

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Bibliographic Details
Published in:ACS Synth Biol
Main Authors: Kim, Seong-Hwan, Lu, Wanli, Ahmadi, Mahmoud Kamal, Montiel, Daniel, Ternei, Melinda A., Brady, Sean F.
Format: Artigo
Language:Inglês
Published: 2018
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6547381/
https://ncbi.nlm.nih.gov/pubmed/30575381
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.8b00361
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