Defining the seed sequence of the Cas12b CRISPR-Cas effector complex

Target binding by CRISPR-Cas ribonucleoprotein effectors is initiated by the recognition of double-stranded PAM motifs by the Cas protein moiety followed by destabilization, localized melting, and interrogation of the target by the guide part of CRISPR RNA moiety. The latter process depends on seed...

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Bibliografiske detaljer
Udgivet i:RNA Biol
Main Authors: Jain, Ishita, Minakhin, Leonid, Mekler, Vladimir, Sitnik, Vasily, Rubanova, Natalia, Severinov, Konstantin, Semenova, Ekaterina
Format: Artigo
Sprog:Inglês
Udgivet: Taylor & Francis 2018
Fag:
Research Paper
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6546406/
https://ncbi.nlm.nih.gov/pubmed/30022698
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15476286.2018.1495492
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