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Mechanism of duplex DNA destabilization by RNA-guided Cas9 nuclease during target interrogation
The prokaryotic clustered regularly interspaced short palindromic repeats (CRISPR)-associated 9 (Cas9) endonuclease cleaves double-stranded DNA sequences specified by guide RNA molecules and flanked by a protospacer adjacent motif (PAM) and is widely used for genome editing in various organisms. The...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5448204/ https://ncbi.nlm.nih.gov/pubmed/28484024 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1619926114 |
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