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Mechanism of duplex DNA destabilization by RNA-guided Cas9 nuclease during target interrogation

The prokaryotic clustered regularly interspaced short palindromic repeats (CRISPR)-associated 9 (Cas9) endonuclease cleaves double-stranded DNA sequences specified by guide RNA molecules and flanked by a protospacer adjacent motif (PAM) and is widely used for genome editing in various organisms. The...

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Bibliografische gegevens
Gepubliceerd in:Proc Natl Acad Sci U S A
Hoofdauteurs: Mekler, Vladimir, Minakhin, Leonid, Severinov, Konstantin
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: National Academy of Sciences 2017
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5448204/
https://ncbi.nlm.nih.gov/pubmed/28484024
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1619926114
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