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An overview of designing and selection of sgRNAs for precise genome editing by the CRISPR-Cas9 system in plants

A large number of computational tools have been documented in recent years for identification of target-specific valid single-guide (sg) RNAs (18–20 nucleotide long sequence) that is an important component for the efficient utilization of the CRISPR-Cas9 (clustered regularly interspaced short palind...

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Détails bibliographiques
Publié dans:3 Biotech
Auteurs principaux: Uniyal, Ajay Prakash, Mansotra, Komal, Yadav, Sudesh Kumar, Kumar, Vinay
Format: Artigo
Langue:Inglês
Publié: Springer International Publishing 2019
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6529479/
https://ncbi.nlm.nih.gov/pubmed/31139538
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-019-1760-2
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