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Mechanism of 5′ splice site transfer for human spliceosome activation

The prespliceosome, comprising U1 and U2 snRNPs bound to the pre-mRNA 5′ splice site (5′SS) and branch point sequence, associates with the U4/U6.U5 tri-snRNP to form the fully-assembled precatalytic pre-B spliceosome. Here, we report cryo-EM structures of the human pre-B complex captured before U1 s...

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Publicat a:Science
Autors principals: Charenton, Clément, Wilkinson, Max E., Nagai, Kiyoshi
Format: Artigo
Idioma:Inglês
Publicat: 2019
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6525098/
https://ncbi.nlm.nih.gov/pubmed/30975767
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.aax3289
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