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Directed Evolution of a Designer Enzyme Featuring an Unnatural Catalytic Amino Acid

The impressive rate accelerations that enzymes display in nature often result from boosting the inherent catalytic activities of side chains by their precise positioning inside a protein binding pocket. Such fine‐tuning is also possible for catalytic unnatural amino acids. Specifically, the directed...

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Detalhes bibliográficos
Publicado no:Angew Chem Int Ed Engl
Main Authors: Mayer, Clemens, Dulson, Christopher, Reddem, Eswar, Thunnissen, Andy‐Mark W. H., Roelfes, Gerard
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6519144/
https://ncbi.nlm.nih.gov/pubmed/30575260
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201813499
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