Caricamento...

Unified energetics analysis unravels SpCas9 cleavage activity for optimal gRNA design

While CRISPR/Cas9 is a powerful tool in genome engineering, the on-target activity and off-target effects of the system widely vary because of the differences in guide RNA (gRNA) sequences and genomic environments. Traditional approaches rely on separate models and parameters to treat on- and off-ta...

Descrizione completa

Salvato in:
Dettagli Bibliografici
Pubblicato in:Proc Natl Acad Sci U S A
Autori principali: Zhang, Dong, Hurst, Travis, Duan, Dongsheng, Chen, Shi-Jie
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2019
Soggetti:
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6500161/
https://ncbi.nlm.nih.gov/pubmed/30988204
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1820523116
Tags: Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne! !