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Commonly used FRET fluorophores promote collapse of an otherwise disordered protein

The dimensions that unfolded proteins, including intrinsically disordered proteins (IDPs), adopt in the absence of denaturant remain controversial. We developed an analysis procedure for small-angle X-ray scattering (SAXS) profiles and used it to demonstrate that even relatively hydrophobic IDPs rem...

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Vydáno v:Proc Natl Acad Sci U S A
Hlavní autoři: Riback, Joshua A., Bowman, Micayla A., Zmyslowski, Adam M., Plaxco, Kevin W., Clark, Patricia L., Sosnick, Tobin R.
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 2019
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6500129/
https://ncbi.nlm.nih.gov/pubmed/30992378
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1813038116
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