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Commonly used FRET fluorophores promote collapse of an otherwise disordered protein

The dimensions that unfolded proteins, including intrinsically disordered proteins (IDPs), adopt in the absence of denaturant remain controversial. We developed an analysis procedure for small-angle X-ray scattering (SAXS) profiles and used it to demonstrate that even relatively hydrophobic IDPs rem...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Riback, Joshua A., Bowman, Micayla A., Zmyslowski, Adam M., Plaxco, Kevin W., Clark, Patricia L., Sosnick, Tobin R.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6500129/
https://ncbi.nlm.nih.gov/pubmed/30992378
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1813038116
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