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In vitro analysis of RNA polymerase II elongation complex dynamics
RNA polymerase II elongation complexes (ECs) were assembled from nuclear extract on immobilized DNA templates and analyzed by quantitative mass spectrometry. Time-course experiments showed that initiation factor TFIIF can remain bound to early ECs, while levels of core elongation factors Spt4–Spt5,...
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| Pubblicato in: | Genes Dev |
|---|---|
| Autori principali: | , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Cold Spring Harbor Laboratory Press
2019
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6499329/ https://ncbi.nlm.nih.gov/pubmed/30846429 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gad.324202.119 |
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