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In vitro assembly and proteomic analysis of RNA polymerase II complexes
The RNA polymerase II (RNApII) transcription cycle consists of multiple steps involving dozens of protein factors. Here we describe a useful approach to study the dynamics of initiation and early elongation, comprising an in vitro transcription system in which complexes are assembled on immobilized...
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| Publicado no: | Methods |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6589106/ https://ncbi.nlm.nih.gov/pubmed/30844430 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2019.03.001 |
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