In vitro analysis of RNA polymerase II elongation complex dynamics

RNA polymerase II elongation complexes (ECs) were assembled from nuclear extract on immobilized DNA templates and analyzed by quantitative mass spectrometry. Time-course experiments showed that initiation factor TFIIF can remain bound to early ECs, while levels of core elongation factors Spt4–Spt5,...

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Detalhes bibliográficos
Publicado no:Genes Dev
Main Authors: Joo, Yoo Jin, Ficarro, Scott B., Chun, Yujin, Marto, Jarrod A., Buratowski, Stephen
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2019
Assuntos:
Research Paper
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6499329/
https://ncbi.nlm.nih.gov/pubmed/30846429
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gad.324202.119
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