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Use of isotopically labeled substrates reveals kinetic differences between human and bacterial serine palmitoyltransferase

Isotope labels are frequently used tools to track metabolites through complex biochemical pathways and to discern the mechanisms of enzyme-catalyzed reactions. Isotopically labeled l-serine is often used to monitor the activity of the first enzyme in sphingolipid biosynthesis, serine palmitoyltransf...

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Detalhes bibliográficos
Publicado no:J Lipid Res
Main Authors: Harrison, Peter J., Gable, Kenneth, Somashekarappa, Niranjanakumari, Kelly, Van, Clarke, David J., Naismith, James H., Dunn, Teresa M., Campopiano, Dominic J.
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6495160/
https://ncbi.nlm.nih.gov/pubmed/30792183
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.M089367
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