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Influence of a 3′ Terminal Ribozyme on AgoshRNA Biogenesis and Activity

Short hairpin RNAs (shRNAs) can induce gene silencing via the RNA interference (RNAi) mechanism. We designed an alternative shRNA molecule with a relatively short base-paired stem that bypasses Dicer and instead is processed by the Argonaute 2 (Ago2) protein into a single guide RNA strand that effec...

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Bibliographic Details
Published in:Mol Ther Nucleic Acids
Main Authors: Herrera-Carrillo, Elena, Gao, Zongliang, Berkhout, Ben
Format: Artigo
Language:Inglês
Published: American Society of Gene & Cell Therapy 2019
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6488825/
https://ncbi.nlm.nih.gov/pubmed/31048184
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.omtn.2019.04.001
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