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sgRNA-shRNA Structure Mediated SNP Site Editing on Porcine IGF2 Gene by CRISPR/StCas9

The SNP within intron 3 of the porcine IGF2 gene (G3072A) plays an important role for muscle growth and fat deposition in pigs. In this study, the StCas9 derived from Streptococcus thermophilus together with the Drosha-mediated sgRNA-shRNA structure were combined to boost the G to A base editing on...

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Bibliografski detalji
Izdano u:Front Genet
Glavni autori: Sun, Yongsen, Yan, Nana, Mu, Lu, Sun, Bing, Deng, Jingrong, Fang, Yuanyuan, Shao, Simin, Yan, Qiang, Han, Furong, Zhang, Zhiying, Xu, Kun
Format: Artigo
Jezik:Inglês
Izdano: Frontiers Media S.A. 2019
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6482158/
https://ncbi.nlm.nih.gov/pubmed/31057603
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fgene.2019.00347
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