Robust CRISPR/Cas9 Genome Editing of the HUDEP-2 Erythroid Precursor Line Using Plasmids and Single-Stranded Oligonucleotide Donors

The study of cellular processes and gene regulation in terminal erythroid development has been greatly facilitated by the generation of an immortalised erythroid cell line derived from Human Umbilical Derived Erythroid Precursors, termed HUDEP-2 cells. The ability to efficiently genome edit HUDEP-2...

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Detalhes bibliográficos
Publicado no:Methods Protoc
Main Authors: Moir-Meyer, Gemma, Cheong, Pak Leng, Olijnik, Aude-Anais, Brown, Jill, Knight, Samantha, King, Andrew, Kurita, Ryo, Nakamura, Yukio, Gibbons, Richard J., Higgs, Douglas R., Buckle, Veronica J., Babbs, Christian
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2018
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Protocol
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6481050/
https://ncbi.nlm.nih.gov/pubmed/31164570
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/mps1030028
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