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Nondestructive, base-resolution sequencing of 5-hydroxymethylcytosine using a DNA deaminase
Here we present APOBEC-Coupled Epigenetic Sequencing (ACE-Seq), a bisulfite-free method for localizing 5-hydroxymethylcytosine (5hmC) at single-base resolution with low DNA input. The method builds upon the observation that AID/APOBEC family DNA deaminase enzymes can potently discriminate between cy...
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| Publicado no: | Nat Biotechnol |
|---|---|
| Main Authors: | , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6453757/ https://ncbi.nlm.nih.gov/pubmed/30295673 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.4204 |
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