NanoBRET: The Bright Future of Proximity-Based Assays

Bioluminescence resonance energy transfer (BRET) is a biophysical technique used to monitor proximity within live cells. BRET exploits the naturally occurring phenomenon of dipole-dipole energy transfer from a donor enzyme (luciferase) to an acceptor fluorophore following enzyme-mediated oxidation o...

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Bibliographic Details
Published in:Front Bioeng Biotechnol
Main Authors: Dale, Natasha C., Johnstone, Elizabeth K. M., White, Carl W., Pfleger, Kevin D. G.
Format: Artigo
Language:Inglês
Published: Frontiers Media S.A. 2019
Subjects:
Bioengineering and Biotechnology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6443706/
https://ncbi.nlm.nih.gov/pubmed/30972335
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fbioe.2019.00056
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