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Kinetic Profile and Molecular Dynamic Studies Show that Y229W Substitution in an NDM-1/L209F Variant Restores the Hydrolytic Activity of the Enzyme toward Penicillins, Cephalosporins, and Carbapenems

The New Delhi metallo-β-lactamase-1 (NDM-1) enzyme is the most common metallo-β-lactamase identified in many Gram-negative bacteria causing severe nosocomial infections. The aim of this study was to focus the attention on non-active-site residues L209 and Y229 of NDM-1 and to investigate their role...

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Detalhes bibliográficos
Publicado no:Antimicrob Agents Chemother
Main Authors: Piccirilli, Alessandra, Brisdelli, Fabrizia, Aschi, Massimiliano, Celenza, Giuseppe, Amicosante, Gianfranco, Perilli, Mariagrazia
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6437508/
https://ncbi.nlm.nih.gov/pubmed/30917978
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.02270-18
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