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A Kinetic Study of the Replacement by Site Saturation Mutagenesis of Residue 119 in NDM-1 Metallo-β-Lactamase

New Delhi metallo-β-lactamase 1 (NDM-1) is a subclass B1 metallo-β-lactamase that exhibits a broad spectrum of activity against β-lactam antibiotics. Here we report the kinetic study of 6 Q119X variants obtained by site-directed mutagenesis of NDM-1. All Q119X variants were able to hydrolyze carbape...

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發表在:Antimicrob Agents Chemother
Main Authors: Marcoccia, Francesca, Mercuri, Paola Sandra, Galleni, Moreno, Celenza, Giuseppe, Amicosante, Gianfranco, Perilli, Mariagrazia
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2018
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC6105831/
https://ncbi.nlm.nih.gov/pubmed/29784851
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.02541-17
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