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Nanobody-based binding assay for the discovery of potent inhibitors of CFTR inhibitory factor (Cif)

Lead identification and optimization are essential steps in the development of a new drug. It requires cost-effective, selective and sensitive chemical tools. Here, we report a novel method using nanobodies that allows the efficient screening for potent ligands. The method is illustrated with the cy...

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Détails bibliographiques
Publié dans:Anal Chim Acta
Auteurs principaux: Vasylieva, Natalia, Kitamura, Seiya, Dong, Jiexian, Barnych, Bogdan, Hvorecny, Kelli L., Madden, Dean R., Gee, Shirley J., Wolan, Dennis W., Morisseau, Christophe, Hammock, Bruce D.
Format: Artigo
Langue:Inglês
Publié: 2019
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6426448/
https://ncbi.nlm.nih.gov/pubmed/30832908
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.aca.2018.12.060
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