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Highly efficient genome editing for single-base substitutions using optimized ssODNs with Cas9-RNPs
Target-specific genome editing using engineered nucleases has become widespread in various fields. Long gene knock-in and single-base substitutions can be performed by homologous recombination (HR), but the efficiency is usually very low. To improve the efficiency of knock-in with single-stranded ol...
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| Publicado no: | Sci Rep |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6423289/ https://ncbi.nlm.nih.gov/pubmed/30886178 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-41121-4 |
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