Live cell monitoring of double strand breaks in S. cerevisiae

We have used two different live-cell fluorescent protein markers to monitor the formation and localization of double-strand breaks (DSBs) in budding yeast. Using GFP derivatives of the Rad51 recombination protein or the Ddc2 checkpoint protein, we find that cells with three site-specific DSBs, on di...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:PLoS Genet
Main Authors: Waterman, David P., Zhou, Felix, Li, Kevin, Lee, Cheng-Sheng, Tsabar, Michael, Eapen, Vinay V., Mazzella, Allison, Haber, James E.
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2019
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6415866/
https://ncbi.nlm.nih.gov/pubmed/30822309
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pgen.1008001
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