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Live cell monitoring of double strand breaks in S. cerevisiae.
We have used two different live-cell fluorescent protein markers to monitor the formation and localization of double-strand breaks (DSBs) in budding yeast. Using GFP derivatives of the Rad51 recombination protein or the Ddc2 checkpoint protein, we find that cells with three site-specific DSBs, on di...
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Main Authors: | , , , , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Public Library of Science (PLoS)
2019-03-01
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Colecção: | PLoS Genetics |
Acesso em linha: | http://europepmc.org/articles/PMC6415866?pdf=render |
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