A carregar...

The Effect of DNA Topology on Observed Rates of R-Loop Formation and DNA Strand Cleavage by CRISPR Cas12a

Here we explored the mechanism of R-loop formation and DNA cleavage by type V CRISPR Cas12a (formerly known as Cpf1). We first used a single-molecule magnetic tweezers (MT) assay to show that R-loop formation by Lachnospiraceae bacterium ND2006 Cas12a is significantly enhanced by negative DNA superc...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Publicado no:Genes (Basel)
Main Authors: van Aelst, Kara, Martínez-Santiago, Carlos J., Cross, Stephen J., Szczelkun, Mark D.
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6409811/
https://ncbi.nlm.nih.gov/pubmed/30813348
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/genes10020169
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!