Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing

Broad use of CRISPR-Cas12a (formerly Cpf1) nucleases(1) has been hindered by the requirement for an extended TTTV protospacer adjacent motif (PAM)(2). To address this limitation, we engineered an enhanced Acidaminococcus sp. Cas12a variant (enAsCas12a) that has a substantially expanded targeting ran...

Fuld beskrivelse

Na minha lista:
Bibliografiske detaljer
Udgivet i:Nat Biotechnol
Main Authors: Kleinstiver, Benjamin P., Sousa, Alexander A., Walton, Russell T., Tak, Y. Esther, Hsu, Jonathan Y., Clement, Kendell, Welch, Moira M., Horng, Joy E., Malagon-Lopez, Jose, Scarfò, Irene, Maus, Marcela V., Pinello, Luca, Aryee, Martin J., Joung, J. Keith
Format: Artigo
Sprog:Inglês
Udgivet: 2019
Fag:
Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6401248/
https://ncbi.nlm.nih.gov/pubmed/30742127
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41587-018-0011-0
Tags: Tilføj Tag
Ingen Tags, Vær først til at tagge denne postø!

Lignende værker