Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing

Broad use of CRISPR-Cas12a (formerly Cpf1) nucleases(1) has been hindered by the requirement for an extended TTTV protospacer adjacent motif (PAM)(2). To address this limitation, we engineered an enhanced Acidaminococcus sp. Cas12a variant (enAsCas12a) that has a substantially expanded targeting ran...

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Detalhes bibliográficos
Publicado no:Nat Biotechnol
Main Authors: Kleinstiver, Benjamin P., Sousa, Alexander A., Walton, Russell T., Tak, Y. Esther, Hsu, Jonathan Y., Clement, Kendell, Welch, Moira M., Horng, Joy E., Malagon-Lopez, Jose, Scarfò, Irene, Maus, Marcela V., Pinello, Luca, Aryee, Martin J., Joung, J. Keith
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6401248/
https://ncbi.nlm.nih.gov/pubmed/30742127
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41587-018-0011-0
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