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Generation of cell-based systems to visualize chromosome damage and translocations in living cells

Traditional methods for the generation of DNA damage are not well suited for the observation of spatiotemporal aspects of damaged chromosomal loci. We describe a protocol for the derivation of a cellular system to induce and to visualize chromosome damage at specific sites of the mammalian genome in...

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Bibliographic Details
Published in:Nat Protoc
Main Authors: Roukos, Vassilis, Burgess, Rebecca C, Misteli, Tom
Format: Artigo
Language:Inglês
Published: 2014
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6391998/
https://ncbi.nlm.nih.gov/pubmed/25255091
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.167
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