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Isothermal Analysis of ThermoFluor Data can readily provide Quantitative Binding Affinities
Differential scanning fluorimetry (DSF), also known as ThermoFluor or Thermal Shift Assay, has become a commonly-used approach for detecting protein-ligand interactions, particularly in the context of fragment screening. Upon binding to a folded protein, most ligands stabilize the protein; thus, obs...
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| Publicado en: | Sci Rep |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Nature Publishing Group UK
2019
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6389909/ https://ncbi.nlm.nih.gov/pubmed/30804351 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-37072-x |
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