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Isothermal Analysis of ThermoFluor Data can readily provide Quantitative Binding Affinities

Differential scanning fluorimetry (DSF), also known as ThermoFluor or Thermal Shift Assay, has become a commonly-used approach for detecting protein-ligand interactions, particularly in the context of fragment screening. Upon binding to a folded protein, most ligands stabilize the protein; thus, obs...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Bai, Nan, Roder, Heinrich, Dickson, Alex, Karanicolas, John
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2019
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6389909/
https://ncbi.nlm.nih.gov/pubmed/30804351
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-37072-x
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