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Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity

Isothermal titration calorimetry (ITC) is a useful tool for understanding the complete thermodynamic picture of a binding reaction. In biological sciences, macromolecular interactions are essential in understanding the machinery of the cell. Experimental conditions, such as buffer and temperature, c...

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Detalhes bibliográficos
Main Authors: Duff,, Michael R., Grubbs, Jordan, Howell, Elizabeth E.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3230191/
https://ncbi.nlm.nih.gov/pubmed/21931288
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/2796
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