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Combination of isothermal titration calorimetry and time-resolved luminescence for high affinity antibody-ligand interaction thermodynamics and kinetics
For experiments using synthetic ligands as probes for biological experiments, it is useful to determine the specificity and affinity of the ligands for their receptors. As ligands with higher affinities are developed (K(A) >10(8) M(−1); K(D) <10(−8) M), a new challenge arises: to measure these...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3294027/ https://ncbi.nlm.nih.gov/pubmed/21964396 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2011.09.011 |
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