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Optimization of protein buffer cocktails using Thermofluor

The stability and homogeneity of a protein sample is strongly influenced by the composition of the buffer that the protein is in. A quick and easy approach to identify a buffer composition which increases the stability and possibly the conformational homogeneity of a protein sample is the fluorescen...

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Detalhes bibliográficos
Main Authors: Reinhard, Linda, Mayerhofer, Hubert, Geerlof, Arie, Mueller-Dieckmann, Jochen, Weiss, Manfred S.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3564630/
https://ncbi.nlm.nih.gov/pubmed/23385769
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112051858
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