Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the small subunit of isopropylmalate isomerase (Rv2987c) from Mycobacterium tuberculosis

Two C-terminally truncated variants of the small subunit of Mycobacterium tuberculosis isopropylmalate isomerase (Rv2987c; LeuD), LeuD_1-156 and LeuD_1-168, have been cloned, heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized. The crysta...

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Detalhes bibliográficos
Main Authors: Karuppasamy, Manikandan, Geerlof, Arie, Schuldt, Linda, Mueller-Dieckmann, Christoph, Weiss, Manfred S.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2009
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2635852/
https://ncbi.nlm.nih.gov/pubmed/19194004
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108042516
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