Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DapB (Rv2773c) from Mycobacterium tuberculosis

Dihydrodipicolinate reductase from Mycobacterium tuberculosis (DapB, DHDPR, Rv2773c) has been cloned and heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized in three different crystal forms. Preliminary diffraction data analysis suggests...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Kefala, Georgia, Janowski, Robert, Panjikar, Santosh, Mueller-Dieckmann, Christoph, Weiss, Manfred S.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: International Union of Crystallography 2005
Pynciau:
Crystallization Communications
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC1952461/
https://ncbi.nlm.nih.gov/pubmed/16511139
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910501938X
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