Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DapC (Rv0858c) from Mycobacterium tuberculosis

N-Succinyldiaminopimelate aminotransferase from Mycobacterium tuberculosis (DAP-AT; DapC; Rv0858c) has been cloned, heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized in two related crystal forms. Preliminary diffraction data analysis su...

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Autores principales: Weyand, Simone, Kefala, Georgia, Weiss, Manfred S.
Formato: Artigo
Lenguaje:Inglês
Publicado: International Union of Crystallography 2006
Materias:
Crystallization Communications
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2242922/
https://ncbi.nlm.nih.gov/pubmed/16880560
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106026753
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