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Identification of Buffer Conditions for Optimal Thermostability and Solubility of Herpesviral Protein UL37 Using the Thermofluor Assay

Structural and biochemical studies of proteins require high amounts of stable, purified proteins. Protein stability often depends on the buffer composition, which includes pH and concentration of salts or other solutes such as glycerol, hence an efficient method for identifying optimal buffer condit...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Koenigsberg, Andrea L., Pitts, Jared D., Heldwein, Ekaterina E.
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842297/
https://ncbi.nlm.nih.gov/pubmed/33659332
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3662
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