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Identification of Buffer Conditions for Optimal Thermostability and Solubility of Herpesviral Protein UL37 Using the Thermofluor Assay

Structural and biochemical studies of proteins require high amounts of stable, purified proteins. Protein stability often depends on the buffer composition, which includes pH and concentration of salts or other solutes such as glycerol, hence an efficient method for identifying optimal buffer condit...

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Bibliografske podrobnosti
izdano v:Bio Protoc
Main Authors: Koenigsberg, Andrea L., Pitts, Jared D., Heldwein, Ekaterina E.
Format: Artigo
Jezik:Inglês
Izdano: Bio-Protocol 2020
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842297/
https://ncbi.nlm.nih.gov/pubmed/33659332
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3662
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