KRAS genotyping by digital PCR combined with melting curve analysis

Digital PCR (dPCR) has been developed as a method that can quantify nucleic acids more sensitively than real-time PCR. However, dPCR exhibits large fluctuations in the fluorescence intensity of the compartment, resulting in low accuracy. The main cause is most likely due to insufficient PCR. In this...

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Veröffentlicht in:Sci Rep
Hauptverfasser: Tanaka, Junko, Nakagawa, Tatsuo, Shiratori, Akiko, Shimazaki, Yuzuru, Uematsu, Chihiro, Kamahori, Masao, Yokoi, Takahide, Harada, Kunio, Kohara, Yoshinobu
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2019
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6384904/
https://ncbi.nlm.nih.gov/pubmed/30796246
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-38822-1
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