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Detection of Low-Level KRAS Mutations Using PNA-Mediated Asymmetric PCR Clamping and Melting Curve Analysis with Unlabeled Probes

Detection of somatic mutations in clinical cancer specimens is often hampered by excess wild-type DNA. The aim of this study was to develop a simple and economical protocol without using fluorescent probes to detect low-level mutations. In this study, we combined peptide nucleic acid (PNA)-clamping...

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Autori principali: Oh, Ji Eun, Lim, Hee Sun, An, Chang Hyeok, Jeong, Eun Goo, Han, Ji Youn, Lee, Sug Hyung, Yoo, Nam Jin
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Investigative Pathology 2010
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2893625/
https://ncbi.nlm.nih.gov/pubmed/20413678
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2353/jmoldx.2010.090146
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