Characterization of Aberrant Melting Peaks in Unlabeled Probe Assays

An unlabeled probe assay relies on a double-stranded DNA-binding dye to detect and verify target based on amplicon and probe melting. During the development and application of unlabeled probe assays, aberrant melting peaks are sometimes observed that may interfere with assay interpretation. In this...

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Bibliographic Details
Main Authors: Dames, Shale, Margraf, Rebecca L., Pattison, David C., Wittwer, Carl T., Voelkerding, Karl V.
Format: Artigo
Language:Inglês
Published: American Society for Investigative Pathology 2007
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1899421/
https://ncbi.nlm.nih.gov/pubmed/17591927
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2353/jmoldx.2007.060139
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