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Characterization of Aberrant Melting Peaks in Unlabeled Probe Assays

An unlabeled probe assay relies on a double-stranded DNA-binding dye to detect and verify target based on amplicon and probe melting. During the development and application of unlabeled probe assays, aberrant melting peaks are sometimes observed that may interfere with assay interpretation. In this...

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Main Authors: Dames, Shale, Margraf, Rebecca L., Pattison, David C., Wittwer, Carl T., Voelkerding, Karl V.
格式: Artigo
語言:Inglês
出版: American Society for Investigative Pathology 2007
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC1899421/
https://ncbi.nlm.nih.gov/pubmed/17591927
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2353/jmoldx.2007.060139
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