Unlabeled Oligonucleotides as Internal Temperature Controls for Genotyping by Amplicon Melting

Amplicon melting is a closed-tube method for genotyping that does not require probes, real-time analysis, or allele-specific polymerase chain reaction. However, correct differentiation of homozygous mutant and wild-type samples by melting temperature (T(m)) requires high-resolution melting and close...

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Detalhes bibliográficos
Main Authors: Seipp, Michael T., Durtschi, Jacob D., Liew, Michael A., Williams, Jamie, Damjanovich, Kristy, Pont-Kingdon, Genevieve, Lyon, Elaine, Voelkerding, Karl V., Wittwer, Carl T.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Investigative Pathology 2007
Assuntos:
Technical Advance
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1899416/
https://ncbi.nlm.nih.gov/pubmed/17591926
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2353/jmoldx.2007.060136
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