Unlabeled Oligonucleotides as Internal Temperature Controls for Genotyping by Amplicon Melting

Amplicon melting is a closed-tube method for genotyping that does not require probes, real-time analysis, or allele-specific polymerase chain reaction. However, correct differentiation of homozygous mutant and wild-type samples by melting temperature (T(m)) requires high-resolution melting and close...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Seipp, Michael T., Durtschi, Jacob D., Liew, Michael A., Williams, Jamie, Damjanovich, Kristy, Pont-Kingdon, Genevieve, Lyon, Elaine, Voelkerding, Karl V., Wittwer, Carl T.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Investigative Pathology 2007
Schlagworte:
Technical Advance
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1899416/
https://ncbi.nlm.nih.gov/pubmed/17591926
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2353/jmoldx.2007.060136
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!

Ähnliche Einträge