Eprobe Mediated Real-Time PCR Monitoring and Melting Curve Analysis

Real-time monitoring of PCR is one of the most important methods for DNA and RNA detection widely used in research and medical diagnostics. Here we describe a new approach for combined real-time PCR monitoring and melting curve analysis using a 3′ end-blocked Exciton-Controlled Hybridization-sensiti...

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Autors principals: Hanami, Takeshi, Delobel, Diane, Kanamori, Hajime, Tanaka, Yuki, Kimura, Yasumasa, Nakasone, Ayako, Soma, Takahiro, Hayashizaki, Yoshihide, Usui, Kengo, Harbers, Matthias
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2013
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3737233/
https://ncbi.nlm.nih.gov/pubmed/23951046
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0070942
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