Sortase-mediated fluorescent labeling of CRISPR complexes

Fluorescent labeling of proteins is a critical requirement for single-molecule imaging studies. Many protein labeling strategies require harsh conditions or large epitopes that can inactivate the target protein, either by decreasing the protein’s enzymatic activity or by blocking protein–protein int...

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Detalhes bibliográficos
Publicado no:Methods Enzymol
Main Authors: Dillard, Kaylee E., Schaub, Jeffrey M., Brown, Maxwell W., Saifuddin, Fatema A., Xiao, Yibei, Hernandez, Erik, Dahlhauser, Samuel D., Anslyn, Eric V., Ke, Ailong, Finkelstein, Ilya J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6380366/
https://ncbi.nlm.nih.gov/pubmed/30691654
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2018.10.031
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