Massively parallel biophysical analysis of CRISPR-Cas complexes on next generation sequencing chips

CRISPR-Cas nucleoproteins target foreign DNA via base pairing with a crRNA. However, a quantitative description of protein binding and nuclease activation at off-target DNA sequences remains elusive. Here, we describe a chip-hybridized association-mapping platform (CHAMP) that repurposes next-genera...

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Detalhes bibliográficos
Publicado no:Cell
Main Authors: Jung, Cheulhee, Hawkins, John, Jones, Stephen K., Xiao, Yibei, Rybarski, James, Dillard, Kaylee E., Hussmann, Jeffrey, Saifuddin, Fatema A., Savran, Cagri A., Ellington, Andrew D., Ke, Ailong, Press, William H., Finkelstein, Ilya J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5552236/
https://ncbi.nlm.nih.gov/pubmed/28666121
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cell.2017.05.044
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