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Sortase-mediated fluorescent labeling of CRISPR complexes

Fluorescent labeling of proteins is a critical requirement for single-molecule imaging studies. Many protein labeling strategies require harsh conditions or large epitopes that can inactivate the target protein, either by decreasing the protein’s enzymatic activity or by blocking protein–protein int...

詳細記述

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書誌詳細
出版年:Methods Enzymol
主要な著者: Dillard, Kaylee E., Schaub, Jeffrey M., Brown, Maxwell W., Saifuddin, Fatema A., Xiao, Yibei, Hernandez, Erik, Dahlhauser, Samuel D., Anslyn, Eric V., Ke, Ailong, Finkelstein, Ilya J.
フォーマット: Artigo
言語:Inglês
出版事項: 2018
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC6380366/
https://ncbi.nlm.nih.gov/pubmed/30691654
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2018.10.031
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