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Sortase-mediated fluorescent labeling of CRISPR complexes
Fluorescent labeling of proteins is a critical requirement for single-molecule imaging studies. Many protein labeling strategies require harsh conditions or large epitopes that can inactivate the target protein, either by decreasing the protein’s enzymatic activity or by blocking protein–protein int...
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| 出版年: | Methods Enzymol |
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| 主要な著者: | , , , , , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2018
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| 主題: | |
| オンライン・アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6380366/ https://ncbi.nlm.nih.gov/pubmed/30691654 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2018.10.031 |
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