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A Biochemical Approach to Identify Direct MicroRNA Targets
We have recently developed a biochemical approach to isolate miRNA-bound mRNAs and have used this method to identify the genome-wide mRNAs regulated by the tumor suppressor miRNA miR-34a. This method involves transfection of cells with biotinylated miRNA mimics, streptavidin pulldown, RNA isolation,...
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| 發表在: | Methods Mol Biol |
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| Main Authors: | , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2015
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6378880/ https://ncbi.nlm.nih.gov/pubmed/25240884 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-1369-5_3 |
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