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Efficiency and fidelity of T3 DNA ligase in ligase-catalysed oligonucleotide polymerisations

Ligase-catalyzed oligonucleotide polymerisations (LOOPER) can readily generate libraries of diversely-modified nucleic acid polymers, which can be subjected to iterative rounds of in vitro selection to evolve functional activity. While there exist several different DNA ligases, T4 DNA ligase has mos...

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Bibliografiske detaljer
Udgivet i:Org Biomol Chem
Main Authors: Lei, Yi, Washington, Joshua, Hili, Ryan
Format: Artigo
Sprog:Inglês
Udgivet: 2019
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6374181/
https://ncbi.nlm.nih.gov/pubmed/30357247
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c8ob01958d
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