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Efficient assembly of very short oligonucleotides using T4 DNA Ligase

BACKGROUND: In principle, a pre-constructed library of all possible short oligonucleotides could be used to construct many distinct gene sequences. In order to assess the feasibility of such an approach, we characterized T4 DNA Ligase activity on short oligonucleotide substrates and defined conditio...

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書目詳細資料
Main Authors: Horspool, Daniel R, Coope, Robin JN, Holt, Robert A
格式: Artigo
語言:Inglês
出版: BioMed Central 2010
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2994885/
https://ncbi.nlm.nih.gov/pubmed/21062485
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-3-291
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