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Efficient assembly of very short oligonucleotides using T4 DNA Ligase
BACKGROUND: In principle, a pre-constructed library of all possible short oligonucleotides could be used to construct many distinct gene sequences. In order to assess the feasibility of such an approach, we characterized T4 DNA Ligase activity on short oligonucleotide substrates and defined conditio...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2994885/ https://ncbi.nlm.nih.gov/pubmed/21062485 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-3-291 |
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