CRISPR-Directed In Vitro Gene Editing of Plasmid DNA Catalyzed by Cpf1 (Cas12a) Nuclease and a Mammalian Cell-Free Extract

Extraordinary efforts are underway to offer greater versatility and broader applications for CRISPR-directed gene editing. Here, we report the establishment of a system for studying this process in a mammalian cell-free extract prepared from HEK-293 human embryonic kidney cells. A ribonucleoprotein...

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Detalles Bibliográficos
Publicado en:CRISPR J
Autores principales: Sansbury, Brett M., Wagner, Amanda M., Nitzan, Erez, Tarcic, Gabi, Kmiec, Eric B.
Formato: Artigo
Lenguaje:Inglês
Publicado: Mary Ann Liebert, Inc. 2018
Materias:
Research Articles
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6345151/
https://ncbi.nlm.nih.gov/pubmed/30687813
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/crispr.2018.0006
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